Against the backdrop of ecological civilization construction, the integration of multiple regulations, and the transitional period of national land spatial planning reform, how to optimize and coordinate ecological protection and rural revitalization through village scale spatial layout has become a key issue in national land spatial governance. This study takes Bayin Maodao Gacha, a typical village in the ecologically fragile area of the Yellow River Basin in Dengkou, Inner Mongolia, as the research area. Based on the results of the 2020 land use change survey in Dengkou, ArcGIS software spatial analysis function and MCR model are used to conduct suitability zoning of the land and resource utilization functions in the research area. Optimization plans are formulated by overlaying the current situation of land and resource utilization, and technical support and theoretical reference are proposed for the preparation of the "multi planning integration" plan in the research area. Based on the definition of land use functional zones and the current land use status map in the research area, combined with field investigations to confirm the status of the land parcels, the land resources in the village area are divided into seven subcategories: forest ecological land, grassland ecological land, water ecological land, other ecological land, village construction land, agricultural production land, and agricultural production service land. Based on the MCR difference, referring to relevant literature on spatial suitability zoning and the current status of land use in the study area, the study area is divided into five categories: rural development area, farmland protection area, ecological agriculture area, ecological control area, and ecological protection area. This study adheres to the principles of “ecological priority, green development”, “people-oriented, respecting public opinion”, and “conservation and intensification, adapting to local conditions”, and formulates strategies for optimizing the spatial layout of the country. All types of land in rural development zones remain unchanged, and village blank land is set up for future development of villages without occupying arable land; the suitability of land construction in farmland protection areas is second only to that in rural development areas. This area has multiple suitability between construction and development and agricultural production, and is a gathering area for residential land and agricultural production land in villages. According to the principle of prioritizing green development, agricultural production activities should be carried out as much as possible in the region, while coordinating the relationship between agricultural production and living land use. Based on the actual situation, there is no need to adjust the types of land use in the region; the ecological agriculture area is a transitional zone between ecology and production space, and is a key area for agricultural land consolidation and the establishment of farmland protective forests. This optimization will adjust all scattered village construction land in the region to agricultural production land, while keeping other land types unchanged. Considering that human activities can easily affect landscape ecology, but agricultural production land has both ecological and production functions, it is advisable to transform agricultural production modes to minimize ecological and environmental damage; the current land use within the ecological control zone is mostly various types of ecological land, which serves as a barrier to ensure the ecological security of the research area. Optimize the adjustment of village construction landwithin the region to agricultural production land, and other ecological land to forest ecological land, while keeping other land types unchanged. In addition, ecological restoration activities can be carried out in ecologically fragile areas such as sandy and saline alkali land; due to being located within the ecological protection red line, ecological protection and restoration should be the main focus of the ecological protection zone. Agricultural production and villagers' daily activities should be prohibited. Therefore, all agricultural production land, agricultural production service land, and village construction land in the area should be optimized to forest ecological land. After following the optimization strategy for adjustment, the ecological land area of grassland and water remains unchanged, while the ecological land area of forest land increases, and the areas of other types of land decrease. The current status of forest ecological land is 230.52 hm2, with an optimized area of 377.07 hm2, an increase of 146.55 hm2. The current areas of agricultural production land, agricultural production service land, village construction land, and other ecological land are 4 190.52 hm2, 431.86 hm2, 125.7 hm2, and 440.34 hm2, respectively. After optimization, they have decreased by 15.03 hm2, 0.25 hm2, 1.82 hm2, and 129.45 hm2, respectively. There are a total of eight expected and constrained indicators for implementing the indicator system of the "Inner Mongolia Autonomous Region Village Planning Compilation Regulations" and the transmission of higher-level planning in the research area. The optimization results of the MCR model meet the requirements of various control indicators such as the permanent basic farmland protection red line, ecological protection red line, and village construction boundary. Compared with the current situation of land and resource utilization pattern, the regularity and connectivity of the optimized land spatial layout have been significantly improved, effectively solving the problems of fragmented land use and disorderly spatial layout in the research area. The research results can provide reference for the preparation of practical village planning in the study area that integrates multiple regulations.