As a famous world natural heritage site, Jinfo Mountain attracts many tourists with its unique natural landscape and rich biodiversity. Jinfo Mountain’s karst landscape, high-altitude cave system, and rich flora and fauna resources make it an important destination for scientific research and tourism. Jinfo Mountain has great potential for the development of its tourism industry, but it is still facing some challenges. For example, single tourism product, insufficient marketing and inadequate infrastructure limit its tourism attractiveness. The study of climate comfort can provide a reference for the development of diversified tourism products in Jinfo Mountain, such as outdoor activities and ecotourism in seasons with comfortable climate. In addition, the study of climate comfort can also help optimize the arrangement of tourism seasons and attract more tourists to come to visit in the appropriate season. However, with the impact of global climate change, changes in climate comfort have become increasingly significant for tourism. China is a mountainous country, and the systematic evaluation of the thermal comfort of mountain environments is an important part of the future development of mountain tourism. Therefore, an in-depth study of the climate comfort of Jinfo Mountain can help to better understand its natural environmental characteristics and can provide a scientific basis for the development of the tourism industry. In this study, Jinfo Mountain in Chongqing is chosen as the research object, based on the real-time microclimate monitoring data of 14 altitude gradients in Jinfo Mountain in 2019, analyzed the vertical change rules of temperature and relative humidity along the altitude gradient in Jinfo Mountain, and introduced the Temperature Humidity Index to determine the climate comfort at different altitudes, and made an evaluation of the climate suitability of Jinfo Mountain tourism area. The results of the study are as follows: 1) the average annual temperature on the western slope of Jinfo Mountain was 11.72 °C, the average annual relative humidity was 93.61%, and the average annual temperature lapse rate was 0.51 °C/100 m. 2) The altitude changes of daily average temperature, monthly average temperature, and annual average temperature of Jinfo Mountain are stable and have obvious differences. 3) The comfortable period of tourism in Jinfo Mountain is from April to October, of which July to August is the golden period of tourism. The climate comfort of the tourist area has obvious seasonal characteristics, that is, summer is the most suitable, followed by spring and autumn, and winter is the most unsuitable for travel. Jinfo Mountain elevation difference, and local microclimate features obvious, while high forest coverage, in the summer effectively alleviate the Yangtze River Canyon sultry climate brings discomfort, is the golden season of summer tourism travel. In spring and fall, the climate of Jinfo Mountain is more comfortable, suitable for tourism activities to the middle and low altitudes. In winter, the climate of Jinfo Mountain is less comfortable, not suitable for outdoor tourism activities, but tourists can enjoy the snow scenery. This study can provide scientific support for the sustainable development of the tourism industry in Jinfo Mountain in the future. It is of great significance for guiding tourists totravel, developing and perfecting tourism products, and expanding the market of health tourism. The evaluation of tourism climate comfort of Jinfo Mountain is not only applicable to itself, but also has important reference value for other mountain or upland tourism areas with similar geographical conditions, especially for those mountain scenic spots located in warm areas or with high summer temperatures. It will help these scenic spots to better carry out tourism planning and management, and enhance tourism attractiveness and visitors’ experience.